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      When applying a Fluorescence Property in TracePro, the Apply Properties dialog requires input values for the following parameters:

      Quantum Efficiency Peak Molar Extinction Molar Concentration Where can I get more information about what these values represent?

      Synopsis
      Apply Properties - Fluorescence - Quantum Efficiency, Peak Molar Extinction, Molar Concentration

      Solution

      These parameters are applicable to fluids used in the Biomedical industry. For more information, we suggest: Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz

      http://www.amazon.com/Principles-Fluorescence-Spectroscopy-Joseph-Lakowicz/dp/0387312781

      For a solid material, an assessment of the units for these parameters leads to a straightforward conclusion of what values to enter in TracePro.

      • Quantum Efficiency (no units)
      • Peak Molar Extinction (liters/mole*cm)
      • Molar Concentration (moles/liter)

      The units of the product of these 3 values are:

      (no units) * (liters/mole*cm) * (moles/liter) = 1/cm

      In the TracePro Apply Properties / Fluorescence dialog:

      • enter "1" for Quantum Efficiency
      • enter "1" for Molar Concentration
      • enter an absorption coefficient (units of 1/cm) for the Peak Molar Extinction Coefficient